产品货号：
GS4740
中文名称：
重组牛肠激酶轻链(大肠杆菌表达)
英文名称：
Recombinant Bovine Enterokinase Light Chain
产品规格：
100IU
发货周期：
1～3天
产品价格：
询价
General Description
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion.It consists of a disulfide-linked 82～140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35～62kDa light chain which contains the catalytic subunit.Additionally,both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease.EK can specially recognize the amino acid sequence DDDDK,and digest the peptide bond after the lysine residue.rEK was report to be more effective than nature EK in cleaving recombinant proteins.Furthermore,the light chain possesses the whole enzyme activity of EK.rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.
Storage
One year when stored at -20℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 28kDa,a single non-glycosylated polypeptide chain containing 235 amino acids.
Source
Escherichia coli.
Physical Appearance
Sterile liquid.
Formulation
50mM Tris-HCl,pH8.0,0.5M NaCl and 50% glycerol.
Endotoxin
Less than 1 EU/μg of rBoEKL as determined by LAL method.
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 50μg of fusion protein in 16hours to 95% completion at 25℃ in a buffer containing 25mM Tris-HCl,pH7.6,50mM NaCl,and 2mM CaCl2.
相关搜索：重组牛肠激酶轻链(大肠杆菌表达)，EK, Bovine, E.coli-derived，Recombinant Bovine Enterokinase Light Chain
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion.It consists of a disulfide-linked 82～140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35～62kDa light chain which contains the catalytic subunit.Additionally,both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease.EK can specially recognize the amino acid sequence DDDDK,and digest the peptide bond after the lysine residue.rEK was report to be more effective than nature EK in cleaving recombinant proteins.Furthermore,the light chain possesses the whole enzyme activity of EK.rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.
Storage
One year when stored at -20℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 28kDa,a single non-glycosylated polypeptide chain containing 235 amino acids.
Source
Escherichia coli.
Physical Appearance
Sterile liquid.
Formulation
50mM Tris-HCl,pH8.0,0.5M NaCl and 50% glycerol.
Endotoxin
Less than 1 EU/μg of rBoEKL as determined by LAL method.
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 50μg of fusion protein in 16hours to 95% completion at 25℃ in a buffer containing 25mM Tris-HCl,pH7.6,50mM NaCl,and 2mM CaCl2.
相关搜索：重组牛肠激酶轻链(大肠杆菌表达)，EK, Bovine, E.coli-derived，Recombinant Bovine Enterokinase Light Chain